Western Blot Analysis of Neurofilament Proteins (NF-H, NF-M) Expression in Brain Tissue in Alzheimer’s Disease

Alzheimer’s disease (AD) is a neurodegenerative disorder marked by cognitive decline, neuronal loss, and pathological brain alterations, such as the accumulation of amyloid plaques and tau tangles. One of the most significant features of AD pathology is the disruption of the cytoskeleton, particularly neurofilaments (NFs), which are major structural components of neurons. Neurofilaments are classified into three main components: neurofilament heavy chain (NF-H), neurofilament medium chain (NF-M), and neurofilament light chain (NF-L). In this study, we investigate the expression of neurofilament proteins (NF-H and NF-M) in brain tissue from Alzheimer’s disease patients, using Western blot analysis. Our findings suggest that alterations in the expression levels of NF-H and NF-M in the brains of AD patients may contribute to the neurodegenerative process. By comparing the levels of NF-H and NF-M in both AD patients and healthy controls, we aim to provide further insight into their roles in Alzheimer’s disease pathogenesis and the potential of these proteins as biomarkers for the disease.