Western blot analysis of tau (tau) protein expression in brain tissues of patients with Alzheimer’s disease

Alzheimer’s disease (AD) is a neurodegenerative disorder that leads to progressive cognitive decline and memory impairment. Its hallmark pathological features include extracellular amyloid-beta plaques and intracellular neurofibrillary tangles composed of hyperphosphorylated tau protein. Tau, a microtubule-associated protein, is critical for maintaining the structure of the cytoskeleton, but in AD, its aberrant phosphorylation leads to aggregation and neurotoxicity. This study aims to investigate the expression of tau protein in brain tissues of Alzheimer’s disease patients using Western blot analysis, a powerful tool for identifying and quantifying specific proteins. Brain tissue samples from AD patients were compared with age-matched controls to examine differences in tau isoforms, including 3-repeat and 4-repeat tau isoforms, and the presence of hyperphosphorylated tau forms.