Evaluation of ApoB-100 Protein Activity and Expression in Tissues in Hyperlipidaemia Using Western Blot

Hyperlipidaemia is a metabolic disorder characterized by elevated levels of lipids in the bloodstream, contributing significantly to atherosclerosis and cardiovascular diseases. Apolipoprotein B-100 (ApoB-100) is a key structural protein of low-density lipoproteins (LDL), playing a central role in lipid transport and metabolism. Evaluating the expression and activity of ApoB-100 in various tissues provides crucial insight into the pathogenesis and progression of lipid disorders. This study investigates the expression levels of ApoB-100 protein in liver, adipose tissue, and aortic samples of hyperlipidaemic models using Western blotting techniques. The research also assesses correlations between ApoB-100 levels, lipid profiles, and histopathological changes. Results reveal a significant upregulation of ApoB-100 in hepatic and vascular tissues in hyperlipidaemic conditions, suggesting its critical involvement in lipid homeostasis dysregulation. These findings offer potential therapeutic implications for targeting ApoB-100 in lipid-lowering interventions.