Analysis of LXRα (Liver X receptor α) protein in liver cells in hyperlipidaemia by western blot analysis

Liver X receptor alpha (LXRα), a member of the nuclear receptor superfamily, serves as a pivotal regulator of lipid and cholesterol metabolism, playing a crucial role in hepatic lipid homeostasis. In hyperlipidaemia, aberrant regulation of LXRα signaling may exacerbate hepatic steatosis and contribute to metabolic disturbances. This study aims to quantify and compare LXRα protein expression levels in liver cells under normolipidemic and hyperlipidemic conditions using the western blotting technique. Liver tissues from control and high-fat diet-induced hyperlipidaemic rat models were homogenized, proteins extracted, separated by SDSPAGE, and probed with specific antibodies against LXRα. Densitometric analysis normalized to housekeeping protein β-actin revealed significant upregulation of LXRα in hyperlipidaemic samples. These findings provide biochemical insight into the role of LXRα in lipid metabolism disorders and support its potential as a therapeutic target in hyperlipidaemia management.